Electrochemical molecular analysis without nucleic acid amplification
نویسندگان
چکیده
منابع مشابه
Electrical and Electrochemical Monitoring of Nucleic Acid Amplification
Nucleic acid amplification is a gold standard technique for analyzing a tiny amount of nucleotides in molecular biology, clinical diagnostics, food safety, and environmental testing. Electrical and electrochemical monitoring of the amplification process draws attention over conventional optical methods because of the amenability toward point-of-care applications as there is a growing demand for...
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Abstract Background and Objective: Culture, microscopic method is a gold standard method for identification of Lishmania parasite. The use of Molecular methods such as RT- PCR compared to microscopic methods has a higher sensitivity and specificity however, it is not widely used due to its expensive equipment and the time requested. The use of nucleic acid sequence based amplification (NASBA) ...
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Real-time nucleic acid amplification, whereby the amplification rate is used to quantify the initial copy number of target DNA or RNA, has proven highly effective for monitoring pathogen loads. Unfortunately, however, current optical methods are limited to centralized laboratories due to complexity, bulk and cost. In response, recent efforts aim to develop lower-cost, electrochemical real-time ...
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Factors that inhibit the amplification of nucleic acids by PCR are present with target DNAs from many sources. The inhibitors generally act at one or more of three essential points in the reaction in the following ways: they interfere with the cell lysis necessary for extraction of DNA, they interfere by nucleic acid degradation or capture, and they inhibit polymerase activity for amplification...
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ژورنال
عنوان ژورنال: Methods
سال: 2005
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2005.05.008